Автор: Shinichi Nakagawa and Tetsuro Hirose
Название: Nuclear Bodies and Noncoding RNAs: Methods and Protocols
Издательство: Humana Press
Год: 2015
ISBN: 978-1493922529
Серия: Methods in Molecular Biology (Book 1262)
Язык: English
Формат: pdf
Размер: 16,2 mb
Страниц: 351
This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.